Catalyzing transglycosylation using a recombinant host cell overexpressing uridine phosphorylase and purine nucleoside phosphorylase
US7781189B2 · kind B2 · utility
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Key dates
| Filing date | Jan 14, 2005 |
| Grant date | Aug 24, 2010 |
| Priority date | — |
| Expiry date | Nov 6, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel strains of genetically modified prokaryotic micro-organisms capable of expressing polypeptides having the enzyme activity of the enzymes uridine phosphorylase (UdP) and purine nucleoside phosphorylase (PNP) are described; the strains in question can be used, both in the form of whole cells and in the form of crude or purified extracts, to catalyse transglycosylation reactions between a donor nucleoside and an acceptor base with particularly high yields. The associated plasmid vectors are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.