Patent · US Active

Thermostable mutants of pyrroloquinoline quinone dependent glucose dehydrogenase

US7781196B2 · kind B2 · utility

0Cited by

4References

23Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Mara Boenitz-Dulat · Tutzing, DE
Peter Kratzsch · Penzberg, DE
Rainer Schmuck · Benediktbeuern, DE

Key dates

Filing date	Oct 14, 2005
Grant date	Aug 24, 2010
Priority date	—
Expiry date	Sep 7, 2027

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N9/0006
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.