Methods of isolating and amplifying nucleic acids using silanized solid support
US7785787B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 1, 2006 |
| Grant date | Aug 31, 2010 |
| Priority date | — |
| Expiry date | Feb 24, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.