Homogeneous luminescence bioassay
US7790392B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 17, 2006 |
| Grant date | Sep 7, 2010 |
| Priority date | — |
| Expiry date | Nov 29, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A homogenous bioassay including a first group including an acceptor which is a short lifetime fluorescent compound capable of energy transfer, and a second group including a quencher which is capable of energy transfer from an acceptor. The increase or decrease of the acceptor's fluorescence resulting from lengthening or shortening of the distance between the acceptor and quencher is measured. The bioassay also includes a third group including a donor for energy transfer to the acceptor, which donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. The first group includes a tag, the third group includes a binder having a high affinity for binding to the tag. Fluorescence of the acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.