Fusion protein suitable for high efficiency expression and the production method thereof

US7795384B2 · kind B2 · utility

Assignees

• Shanghai Centre of Research & Development of New Drugs · CN
• Shanghai Newsummit Biopharma Co., Ltd · CN
• SHANGHAI AIZHONG BIOTECHNOLOGY CO., LTD. · CN

Inventors

• Yi-Tsung Lu · Shanghai, CN
• Xin Gao · Chengdu, CN
• Dafu Cui · Shanghai, CN
• Youshang Zhang · Shanghai, CN
• Yangbin Huang · Shanghai, CN
• Jiuru Sun · Melbourne, AU
• Jiang Li · Chicago, US
• Jian Fei · Shanghai, CN

Key dates

• Filing date: Jun 3, 2003
• Grant date: Sep 14, 2010
• Expiry date: Oct 5, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a novel fusion protein suitable for high expression and the production method thereof. The structure of the fusion protein is A-C1-K-(B-C2-K)n-D, wherein A is the upstream peptide; each of C1 and C2 is independently short peptides having 20-40 amino acids in length and containing no Lys in sequence; K is Lys; B is a linker; D is a downstream peptide; n is an integer of 3-30. The fusion protein is expressed with high efficiency and stability in host cells. The purification and enzymatic cleavage technology of the expression product is simple, which is able to produce a short peptide with high efficiency, and has very high value of industrialization.