Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
US7795497B2 · kind B2 · utility
5Cited by
5References
10Claims
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Key dates
| Filing date | Dec 19, 2003 |
| Grant date | Sep 14, 2010 |
| Priority date | — |
| Expiry date | Mar 2, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8274
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting a antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.