Specific double-stranded probes for homogeneous detection of nucleic acid and their application methods

US7799522B2 · kind B2 · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Qingge Li · Xiamen, CN
• Jixuan Liang · Fujian, CN
• Guoyan Luan · Fujian, CN

Key dates

• Filing date: Oct 5, 2001
• Grant date: Sep 21, 2010
• Expiry date: Jan 11, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6844
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Double-stranded nucleic acid hybridization probes comprise a longer strand perfectly complementary to a preselected target sequence in an assay and a shorter second strand complementary to the longer strand. The strands are labeled with interactive labels such as a fluorophore and a quencher. The probes may be used in real-time amplification assays to distinguish among alleles.