Affinity peptides and method for purification of recombinant proteins
US7799561B2 · kind B2 · utility
1Cited by
14References
36Claims
0Family size
Assignee
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Key dates
| Filing date | Jun 12, 2003 |
| Grant date | Sep 21, 2010 |
| Priority date | — |
| Expiry date | Dec 28, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K7/08
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
This invention describes a process for separating a fusion protein or polypeptide in the form of its precursor from a mixture containing said fusion protein and impurities, which comprises contacting said fusion protein with a resin containing immobilized metal ions, said fusion protein covalently operably linked directly or indirectly to an immobilized metal ion-affinity peptide, binding said fusion protein to said resin, and selectively eluting said fusion protein from said resin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.