Fluorescence energy transfer by competitive hybridization
US7803528B1 · kind B1 · utility
9Cited by
14References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 26, 1998 |
| Grant date | Sep 28, 2010 |
| Priority date | — |
| Expiry date | Feb 26, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/707
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for detecting the presence of nucleotides or monitoring nucleotide amplification. It utilizes fluorescence energy transfer by competitive hybridization. Competitive hybridization is achieved by using unequal length complementary probes which have a fluorophore on one probe and a quencher on the other. The fluorophore and quencher are juxtaposed in a manner wherein the proximity of the quencher to the fluorophore produces quenching of the fluorescence of the fluorphore.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.