Method of increasing discrimination for single base mismatch using hurdle DNA and artificially mismatched probe
US7803540B2 · kind B2 · utility
1Cited by
2References
7Claims
0Family size
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Key dates
| Filing date | Jan 19, 2006 |
| Grant date | Sep 28, 2010 |
| Priority date | — |
| Expiry date | Jan 19, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of increasing discrimination for a target DNA having a polymorphic site is provided. The method comprising immobilizing first and second probes on a substrate; hybridizing the immobilized first and second probes with first and second hurdle DNAs, respectively; and hybridizing the target DNA with the hybrids, and determining the ratio of a signal of the target DNA hybridized to the first probe to a signal of the target DNA hybridized to the second probe. The addition of a hurdle DNA and variation of a probe base can improve an ability of discriminating a single base mismatch.
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