Protein aggregation regulators
US7803559B1 · kind B1 · utility
5Cited by
3References
18Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 9, 2004 |
| Grant date | Sep 28, 2010 |
| Priority date | — |
| Expiry date | Jul 18, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/2821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is a high-throughput assay to measure intracellular polyglutamine protein aggregation using fluorescence resonance energy transfer (FRET). Three libraries of over 3000 biologically active small molecules were screened for inhibitory activity, and a lead compound was characterized in detail. Y-27632, an inhibitor of the Rho-associated kinase p160ROCK, diminished polyglutamine protein aggregation at micromolar concentrations, and reduced neurodegeneration in a Drosophila model of polyglutamine disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.