Compositions and methods for detecting phosphomonoester
US7803751B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 9, 2005 |
| Grant date | Sep 28, 2010 |
| Priority date | — |
| Expiry date | Sep 19, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/13
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method of modifying a phosphomonoester moiety of a target compound. The method can include the steps of (a) providing a target compound having an electrophilic moiety and a phosphomonoester moiety; (b) contacting the target compound with a first carbodiimide compound under conditions for preferential addition of the first carbodiimide compound to the electrophilic moiety over the phosphomonoester moiety, thereby forming an electrophile-protected target compound; and (c) contacting the electrophile-protected target compound with a second carbodiimide compound and a nucleophilic compound under conditions for addition of the nucleophilic compound to the phosphomonoester.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.