Reagent for fluorimetric determination of analytes

US7807402B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Carina Horn · Biblis, DE
• Hans-Peter Josel · Weilheim, DE
• Juergen Spinke · Lorsch, DE
• Rupert Herrmann · Weilheim, DE
• Dieter Heindl · Munich, DE

Key dates

• Filing date: Apr 22, 2008
• Grant date: Oct 5, 2010
• Expiry date: Apr 22, 2028

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/904
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation.