Patent · US Active

Expression vector system regulated by σ32 and methods for using it to produce recombinant protein

US7807460B2 · kind B2 · utility

0Cited by
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7Claims
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Inventors

Key dates

Filing dateDec 21, 2006
Grant dateOct 5, 2010
Priority date—
Expiry dateDec 21, 2026

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/70
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention discloses an expression vector system comprising a promoter recognized and regulated by a heat shock sigma factor of E. coli, especially σ32. Preferably, the promoter comprises the consensus sequence of E. coli heat shock promoters as shown in SEQ ID NO:1. Also disclosed are methods for producing proteins using the promoter under heat shock conditions. Furthermore, the present invention discloses a method for creating a sudden temperature shift in a cell culture which has been pre-cultured to reach an optimal condition and which temperature shift will allow optimal production of a recombinant protein under the control a heat shock promoter.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.