Fluorescence polarization-based homogenous assay for aflatoxins
US7811775B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 27, 2008 |
| Grant date | Oct 12, 2010 |
| Priority date | — |
| Expiry date | May 27, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.