Method of forming dendritic cells from embryonic stem cells
US7811821B2 · kind B2 · utility
6Cited by
2References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 31, 2006 |
| Grant date | Oct 12, 2010 |
| Priority date | — |
| Expiry date | Mar 9, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/02
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.