System and method for processing specimens and images for optical tomography
US7811825B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 13, 2004 |
| Grant date | Oct 12, 2010 |
| Priority date | — |
| Expiry date | Dec 25, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/365
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a stopped-flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.