Fluorescence detection
US7812324B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 17, 2005 |
| Grant date | Oct 12, 2010 |
| Priority date | — |
| Expiry date | Sep 11, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6408
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence detection system comprises a light source (22), dichroic mirror (32), excitation port (16), emission port (14), and a detector. The light source (22) is, for example, a pulsed ultraviolet LED, with a light emission that decays sufficiently rapidly to permit gated detection of fluorescence from a fluorescently-labelled species, at a time when it is distinguishable from autofluorescence. The detector is, for example, an electron multiplying CCD, with high gain on-chip amplification. A circuit (26) may be used to control a repeating cycle of (i) generation of a 20-200 microsecond UV. pulse; (ii) a gate delay of 1-5 microseconds; and (iii) a 10-800 microsecond detection period. This allows time-resolved-fluorescence-microscopy with real time or near real time operation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.