Method of nonlinear harmonic holography
US7813016B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2007 |
| Grant date | Oct 12, 2010 |
| Priority date | — |
| Expiry date | Nov 6, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG03H2240/56
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A harmonic holography (H2) technique and system that combines holography and nonlinear optics that enables holographic recording of 3D images with femtosecond framing time are provided. The H2 technique records holograms with second harmonic (SH) signals scattered off specialized nanocrystals that are functionalized to label specific protein or other biomolecules in a living organism. The capability of generating second harmonic radiations is specific to materials with noncentrosymmetric crystalline structures only, and χ(2) vanishes for all other types of materials. Therefore, a sharp contrast is formed when particles of noncentrosymmetric structures are dispersed in a medium of other species, pumped at a fundamental frequency, and imaged at the second harmonic frequency. The new scheme described herein provides a sound basis for a new type of contrast microscopy with enormous potential in molecular biomedical imaging.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.