Patent · US Active

Preparation of samples for LC-MS/MS using magnetic particles

US7815803B2 · kind B2 · utility

4Cited by

2References

11Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Uwe Kobold · Wielenbach, DE
Albert Geiger · Fort Saskatchewan, CA
Rupert Herrmann · Weilheim, DE
Michael Vogeser · Munich, DE

Key dates

Filing date	Jun 5, 2008
Grant date	Oct 19, 2010
Priority date	—
Expiry date	Apr 18, 2029

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/6842
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.