Methods for producing Factor VIII proteins
US7820796B2 · kind B2 · utility
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62Claims
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Key dates
| Filing date | Jan 26, 2005 |
| Grant date | Oct 26, 2010 |
| Priority date | — |
| Expiry date | Jan 26, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/755
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for purification of Factor VIII polypeptides by affinity chromatography and ion exchange chromatography, in which the eluate from the affinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The affinity matrix may comprise a monoclonal antibody or a peptide ligand. The methods result in improved purification without significant yield loss.
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