Methods of purifying Fc region containing proteins
US7820799B2 · kind B2 · utility
37Cited by
49References
86Claims
0Family size
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Key dates
| Filing date | Jun 16, 2006 |
| Grant date | Oct 26, 2010 |
| Priority date | — |
| Expiry date | Oct 25, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6854
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides. The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.