Highly purified proteinase K

US7820801B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Helmut Schoen · Penzberg, DE
• Guenter Guertler · Penzberg, DE
• Bernhard Rexer · Weilheim, DE
• Johann-Peter Thalhofer · Weilheim, DE

Key dates

• Filing date: Sep 29, 2006
• Grant date: Oct 26, 2010
• Expiry date: Jun 4, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C11D3/38609
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for producing solid proteinase K which is insoluble in water and has high purity, the method comprising the steps of adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid, and isolating the solid proteinase K.