Non-uniform electric field chamber for cell fusion
US7824901B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 1, 2003 |
| Grant date | Nov 2, 2010 |
| Priority date | — |
| Expiry date | Feb 12, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M35/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An apparatus is provided for carrying out fusion of biological cells (10) and includes a base member (24) on which a conductive outer electrode (18) is supported and has an outer electrode radius (r2) and has an electrode height (19). A conductive inner electrode (20) is supported on the base member (24) and has an inner electrode radius (r1) and also has the electrode height (19). The outer and inner electrodes (18,20) are spaced apart from each other by a gap which defines a fusion chamber (14). The inner electrode radius (r1), the outer electrode radius (r2), and the gap are selected in accordance with a predetermined range of selectable ratios (r1/r2) in a range from 0.7 to 0.9, wherein a selected gap is limited by the range of selectable ratios (r1/r2), and wherein a determined ratio (r1/r2) among the selectable ratios is based on the selected gap, such that compression between the biological cells (10) and permeability between cell membranes are maximized and temperature rise is minimized for providing cell fusion in the fusion chamber (14).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.