Correction method and measurement device for anti-stokes photoluminescence measurement
US7826052B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 2006 |
| Grant date | Nov 2, 2010 |
| Priority date | — |
| Expiry date | Apr 11, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method to correct measurement error in a resonance energy-transfer assay, including exciting anti-Stokes photoluminescent donors with at least one wavelength of light which is greater than an emission wavelength of acceptor molecules; measuring emission at the acceptor's emission wavelength and which differs from the donor's emission wavelength in at least two different time windows; a first time window within the time window defined by the excitation light pulse and a second non-overlapping time window which follows the first time window; and correcting the emission signal, which includes signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission signal measured in the second time window.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.