Polynucleotide barcoding

US7829278B2 · kind B2 · utility

Assignees

• BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS · US
• The Regents of the University of California · US

Inventors

• Paul R. Selvin · Berkeley, US
• Matthew T. Gordon · Urbana, US
• Pui-Yan Kwok · Clayton, US
• Ming Xiao · Huntingdon Valley, US
• Ting-Fung Chan · San Francisco, US

Key dates

• Filing date: Oct 28, 2004
• Grant date: Nov 9, 2010
• Expiry date: Aug 21, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.