High-pressure inclusion body solubilization and protease clipping of recombinant fusion proteins
US7829681B2 · kind B2 · utility
8Cited by
1References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 7, 2008 |
| Grant date | Nov 9, 2010 |
| Priority date | — |
| Expiry date | May 28, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.