High throughput methods for sampling seeds
US7832143B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 23, 2009 |
| Grant date | Nov 16, 2010 |
| Priority date | — |
| Expiry date | Jun 21, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
A high-throughput method is provided for analyzing individual seeds in a population of seeds. The method generally includes removing a tissue sample from at least one or more seeds in the population of seeds while preserving germination viability of the at least one or more seeds from which the tissue sample is taken, depositing the tissue sample into an individual compartment of a sample tray, contacting the tissue sample with an extraction buffer to remove DNA from the sample, and analyzing the extracted DNA for the presence or absence of a transgene of interest. The extraction buffer may be present in the individual compartment of the sample tray before the tissue sample is deposited into the individual compartment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.