Biomolecules having multiple attachment moieties for binding to a substrate surface

US7833715B1 · kind B1 · utility

Assignee

• Nanogen Recognomics GmbH · DE

Inventors

• Markus Schweitzer · Frankfurt am Main, DE
• Norbert Windhab · Hofheim, DE
• John Havens · San Diego, US
• Thomas J. Onofrey · San Marcos, US
• Charles Greef · Louisville, US
• Daguang Wang · White Plains, US

Key dates

• Filing date: Jan 18, 2007
• Grant date: Nov 16, 2010
• Expiry date: Jun 24, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54353
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.