Tagged oligonucleotides and their use in nucleic acid amplification methods
US7833716B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 2007 |
| Grant date | Nov 16, 2010 |
| Priority date | — |
| Expiry date | Jan 8, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6895
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for selective amplification of at least one target nucleic acid sequence, comprising the steps of: treating a sample with a tagged oligonucleotide comprising a target hybridizing sequence that hybridizes to a 3′-end of the target nucleic acid sequence, and a tag sequence situated 5′ to the target hybridizing sequence that does not stably hybridize to a target nucleic acid, wherein tagged oligonucleotide hybridized to target nucleic acids form tagged target nucleic acids; prior to initiating a primer extension reaction, reducing the effective concentration of unhybridized tagged oligonucleotide having an active form; initiating an extension reaction to produce a primer extension product; separating the primer extension product from the target nucleic acid; and producing amplification products therefrom using an oligonucleotide that hybridizes to the complement of the tag sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.