Patent · US Active

McrA variants and uses thereof

US7833769B2 · kind B2 · utility

2Cited by
0References
7Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 27, 2005
Grant dateNov 16, 2010
Priority date—
Expiry dateMar 9, 2027

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/143333
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Compositions and methods are provided in which the composition is a protein with at least 50% but less than 100% amino acid sequence identity with McrA or is a variant McrA protein with at least one amino acid sequence modification. The variant or protein has the property of cleaving DNA with methylated cytosine and not hydroxymethylated cytosine in a target DNA sequence, or substantially lacks catalytic activity while maintaining binding activity. Methods are provided in which the protein or McrA variant are used to identify methylation sites either by cleavage or by binding to the methylation site in the presence of a marker or by binding to an immobilized protein or McrA variant.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.