Combination of insulin and ascorbate to enhance wound healing
US7834153B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 5, 2007 |
| Grant date | Nov 16, 2010 |
| Priority date | — |
| Expiry date | Oct 29, 2028 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K35/28
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided is a method of stimulating collagen synthesis and proteoglycan (lumican and keratocan) accumulation. Collagenase isolated keratocytes were cultured with or without insulin with or without ascorbate. Insulin stimulates the synthesis of collagen but does not affect the accumulation of lumican and keratocan. Insulin plus ascorbate, however, stimulates the synthesis of collagen and increased the accumulation of these proteoglycans. The accumulation of PGDS, a KSPG that does not interact with collagen, is not affected by ascorbate. Only the collagen made in the presence of ascorbate was pepsin resistant. EDB overrode the effects of ascorbate on pepsin resistance and proteoglycan accumulation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.