Phospholipases and uses thereof

US7838274B2 · kind B2 · utility

Assignee

• DSM IP ASSETS B.V. · NL

Inventors

• Kaj Albermann · Martinsried, DE
• Wolfram Kemmner · Munich, DE
• Dieter Maier · Martinsried, DE
• Fabio Spreafico · Martinsried, DE
• Alexander Stock · Martinsried, DE
• Christian Wagner · Martinsried, DE
• Lex De Boer · Wateringen, NL
• Roelf Bernhard Meima · Woerden, NL

Key dates

• Filing date: Aug 6, 2009
• Grant date: Nov 23, 2010
• Expiry date: Aug 6, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel phospholipase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a phospholipase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.