Peptide derivatization method to increase fragmentation information from MS/MS spectra
US7838303B2 · kind B2 · utility
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Key dates
| Filing date | Jul 27, 2006 |
| Grant date | Nov 23, 2010 |
| Priority date | — |
| Expiry date | Apr 12, 2029 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of facilitating analysis of a peptide in a mass spectrometer comprising derivatizing the C-terminus of the peptide with an amino acid residue via a reaction with a carbodiimide reagent, yielding a derivative peptide, ionizing the derivative peptide with a double charge, and fragmenting the ionized derivative peptide in a mass spectrometry system, wherein binary fragments of the ionized derivative each include a charge, facilitating sequence analysis of the peptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.