Recombinant canine thyroid stimulating hormone and methods of production and use thereof
US7838492B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jan 9, 2009 |
| Grant date | Nov 23, 2010 |
| Priority date | — |
| Expiry date | May 2, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P5/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention includes a nucleic acid having a sequence at least 98% homologous to SEQ ID NO: 1, which encodes the α subunit of canine thyroid stimulating hormone (TSH). The invention also includes a nucleic acid having a sequence at least 98% homologous to SEQ ID NO: 2, which encodes the β subunit of canine TSH. The invention also includes a method of producing a recombinant canine thyroid stimulating hormone (rcTSH) subunit by expressing a nucleic acid having a sequence of SEQ ID NO: 1 and a nucleic acid having a sequence of SEQ ID NO: 2 in a transgenic insect cell modified to sialylate proteins and producing a sialylated rcTSH subunit. The insect cell may be a lepidopteran cell. The rcTSH may be used for diagnosis and treatment. It may be used to diagnose canine hypothyroidism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.