Herpesvirus ribozymes and vectors

US7838659B2 · kind B2 · utility

Assignee

• University of Florida Research Foundation, INC. · US

Inventors

• Alfred S. Lewin · Gainesville, US
• David C. Bloom · Gainesville, US
• Gregory S. Schultz · Gainesville, US
• Sonal Sanjeev Tuli · Gainesville, US
• Jia Liu · Boca Raton, US

Key dates

• Filing date: Feb 25, 2008
• Grant date: Nov 23, 2010
• Expiry date: Sep 11, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/16622
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Hammerhead ribozymes that target components critical to HSV replication (ICP4, UL20, UL30, and UL54) were synthesized and shown to efficiently cleave target RNA encoding a portion of these components in in vitro assays. Several cloned cell lines stably expressing these ribozymes were established. HSV-mediated plaque formation was dramatically reduced in cells stably transfected with an anti-HSV ribozyme compared to non-transfected cells.