Fluorogenic probes for reactive oxygen species
US7842823B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 27, 2006 |
| Grant date | Nov 30, 2010 |
| Priority date | — |
| Expiry date | Jan 10, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/84
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a novel class of fluorogenic probes for reactive oxygen species. Exemplary probes of the invention utilize a boronate deprotection mechanism to provide high selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species (ROS) including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical; Peroxyresorufin-1 (PR1), Peroxyfluor-1 (PF1), and Peroxyxanthone-1 (PX1) are first-generation probes that respond to H2O2 by an increase in red, green, and blue fluorescence, respectively. The boronate dyes are cell-permeable and can detect micromolar changes in H2O2 concentrations in living cells, including hippocampal neurons, using confocal and two-photon microscopy. The unique combination of ROS selectivity, membrane permeability, and a range of available excitation/emission colors establishes the potential value of PR1, PF1, PX1, and related probes for interrogating the physiology and pathology of cellular H2O2.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.