Calibrating microarrays
US7846713B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 10, 2002 |
| Grant date | Dec 7, 2010 |
| Priority date | — |
| Expiry date | Jul 13, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay for measuring the amount of a first analyte in a sample, comprises the steps of: (i) contacting the sample with a device that comprises one or more first reaction sites which comprise a first ligand having affinity for the first analyte, and a series of second reaction sites each comprising different known concentrations of an immobilized second analyte; (ii) removing any unbound first analyte; (iii) contacting the device with a second ligand that is detectably labelled and which has affinity for the first analyte, and a third ligand that is detectably labelled and which has affinity for the second analyte; (iv) removing any unbound second and third ligands; and (v) measuring the amount of second and third ligands, wherein measurement of the third ligand is used to establish a calibration curve, used to determine the amount of first analyte present in sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.