Genes encoding a nystatin polyketide synthase and their manipulation and utility

US7851183B2 · kind B2 · utility

Assignee

• Sinvent AS · NO

Inventors

• Sergey Zotchev · Trondheim, NO
• Olga Nikolayivna Sekurova · Trondheim, NO
• Espen Fjærvik · Trondheim, NO
• Trygve Brautaset · Trondheim, NO
• Arne Reidar Strøm · Trondheim, NO
• Svein Valla · Vikhammer, NO
• Trond Erling Ellingsen · Trondheim, NO
• Havard Sletta · Trondheim, NO
• Ole-Martin Gulliksen · Oslo, NO

Key dates

• Filing date: Mar 29, 2010
• Grant date: Dec 14, 2010
• Expiry date: Mar 29, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/62
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 35; or (b) a nucleotide sequence which is the complement of SEQ ID No. 35; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 35; or (d) a nucleotide sequence hybridising under conditions of high stringency to SEQ ID No. 35, to the complement of SEQ ID No. 35, or to a hybridisation probe derived from SEQ ID No. 35 or the complement thereof; or (e) a nucleotide sequence having at least 80% sequence identity with SEQ ID No. 35; or (f) a nucleotide sequence having at least 65% sequence identity with SEQ ID No. 35 wherein said sequence preferably encodes or is complementary to a sequence encoding a nystatin PKS enzyme or a part thereof. Also provided are part of such molecules and polypeptides (and parts thereof) encoded by such a nucleic acid molecule, and the use of such molecules and polypeptides in facilitating nystatin biosynthesis and in the synthesis of nystatin derivatives and novel polyketide as macrolide structures.