Reduction of the hook effect in assay devices
US7851209B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 3, 2003 |
| Grant date | Dec 14, 2010 |
| Priority date | — |
| Expiry date | Mar 24, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54393
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes conjugated probes that contain a specific binding member for the analyte of interest. The specific binding member preferentially complexes with the analyte within a test sample when contacted therewith. Excess analyte that remains uncomplexed with the specific binding member undergoes non-specific binding, such as to a hydrophobic domain. As a result, the ability of the uncomplexed analyte to compete with the complexed analyte at the detection zone of the device is restricted. Thus, the incidence of “false negatives” is limited in a simple, efficient, and relatively inexpensive manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.