Method for carrying out an electrochemical measurement on a liquid measuring sample in a measuring chamber that can be accessed by lines, and corresponding arrangement

US7851227B2 · kind B2 · utility

Assignee

• Siemens Aktiengesellschaft · DE

Inventors

• Heike Barlag · Koblenzer Straße, DE
• Siegfried Birkle · Höchstadt an der Aisch, DE
• Walter Gumbrecht · Herzogenaurach, DE
• Daniela Kühn · Hemhofen, DE
• Peter Paulicka · Erlangen, DE
• Manfred Stanzel · Erlangen, DE

Key dates

• Filing date: Oct 17, 2005
• Grant date: Dec 14, 2010
• Expiry date: Jan 16, 2027

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

Especially in order to carry out the so-called enzyme-coupled DNA hybridization test in a closed cartridge including a microfluid system, using stored dry reagents, the reagents must be dissolved in the microfluid system and transported into the measuring chamber directly before the measurement. During the dissolution of the reagents in water, air cushions that cannot reach the measuring chamber must absolutely be prevented from forming upstream of the reagent liquid. According to an embodiment of the invention, the liquid measuring sample and the liquid reagents are transported in such a way that the air cushion is directed into the waste line and the measuring sample and the reagents are then introduced into the measuring chamber without any air bubbles. In this way, measuring errors can be avoided.