Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
US7855069B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 19, 2005 |
| Grant date | Dec 21, 2010 |
| Priority date | — |
| Expiry date | Nov 20, 2026 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.