Integrated portable biological detection system
US7857957B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 2007 |
| Grant date | Dec 28, 2010 |
| Priority date | — |
| Expiry date | Jan 28, 2029 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0487
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.