Patent · US Active

Fluorogenic enzyme substrates and methods of preparation

US7863428B2 · kind B2 · utility

0Cited by

3References

5Claims

0Family size

Assignee

Tibotec BVBA · BE

Inventors

Inge Dierynck · Berchem, BE
Jan Ludwig Goeman · Sint-Niklaas, BE
Koenraad Lodewijk August Van Acker · Temse, BE
Johan Van Der Eycken · Ninove, BE

Key dates

Filing date	Dec 26, 2003
Grant date	Jan 4, 2011
Priority date	—
Expiry date	Aug 23, 2026

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07H15/00
WIPO fieldOrganic fine chemistry
WIPO sectorChemistry

Abstract

This invention relates to hydrolase fluorogenic substrates with improved cell permeability, methods for the preparation thereof, and methods of measuring activities of hydrolases, particularly in cell-based assays. The substrates easily diffuse into the cells, where they are enzymatically processed to yield photostable fluorescent products, and are particularly fitted for visualising enzyme-derived activities in cell-based assays.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.