Construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences

US7863430B2 · kind B2 · utility

Assignee

• Queensland University of Technology · AU

Inventors

• James Langham Dale · Ipswich, AU
• Benjamin Dugdale · Brisbane, AU
• Greg John Hafner · Pinnaroo, AU
• Scott Richard Hermann · Redcliffe, AU
• Douglas Kenneth Becker · Dajarra, AU
• Robert Maxwell Harding · Las Vegas, US
• Srimek Chowpongpang · Samut, TH

Key dates

• Filing date: Mar 28, 2001
• Grant date: Jan 4, 2011
• Expiry date: Apr 29, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8283
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates generally to constructs and in particular genetic constructs comprising polynucleotide sequences capable of release in covalently closed, circular form from a larger nucleotide sequence such as a genome of a eukaryotic cell. Preferably, once released, a polynucleotide sequence is reconstituted in a form which permits expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence with all or part of an extraneous nucleotide such as an intronic sequence or other splice signal inserted therein. Expression and in particular transcription of the coding sequence involves splicing out the extraneous sequence. The release and circularization is generally in response to a stimulus such as a protein-mediated stimulus. More particularly, the protein is a viral or prokaryotic or eukaryotic derived protein or developmentally and/or tissue specific regulated protein.