Capture and detection of target nucleic acid in dipstick assays
US7867706B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 2007 |
| Grant date | Jan 11, 2011 |
| Priority date | — |
| Expiry date | Jun 2, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/94
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.