Bioluminogenic assay system for measuring beta-lactamase activity

US7867758B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Jianghong Rao · Sunnyvale, US
• Min-kyung So · Palo Alto, US
• Hequan Yao · Nanjing, CN

Key dates

• Filing date: Apr 1, 2008
• Grant date: Jan 11, 2011
• Expiry date: Jul 2, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/986
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A bioluminogenic assay system including: providing a bioluminogenic substrate incorporating a beta-lactam antibiotic, a bioluminescence initiating compound, and a chemical linkage joining the beta-lactam antibiotic to the bioluminescence initiating compound; exposing the bioluminogenic substrate to a beta-lactamase enzyme that catalyzes the release of the bioluminescence initiating compound from the bioluminogenic substrate; co-exposing the bioluminogenic substrate to a bioluminescence indicator reaction that employs the bioluminescence initiating compound as a substrate; and detecting a light from the bioluminescence indicator reaction as a measure of the activity of the beta-lactamase enzyme.