Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US7868138B2 · kind B2 · utility
143Cited by
105References
6Claims
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Key dates
| Filing date | Feb 1, 2008 |
| Grant date | Jan 11, 2011 |
| Priority date | — |
| Expiry date | Apr 27, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.