Polynucleotides encoding anti-sulfotyrosine antibodies

US7868152B2 · kind B2 · utility

Assignee

• LOS ALAMOS NATIONAL SECURITY, LLC · US

Inventors

• Carolyn R. Bertozzi · Berkeley, US
• John Kehoe · Schaumburg, US
• Andrew M. Bradbury · Anthony, US

Key dates

• Filing date: Sep 15, 2009
• Grant date: Jan 11, 2011
• Expiry date: Sep 15, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/622
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.