Method for eliminating crosstalk between waveguide grating-based biosensors located in a microplate and the resulting microplate

US7871811B2 · kind B2 · utility

Assignee

• CORNING INCORPORATED · US

Inventors

• Ye Fang · Corning, US
• Ann MeeJin Ferrie · Corning, US
• Norman Henry Fontaine · Corning, US
• Anthony G. Frutos · Corning, US
• Eric John Mozdy · Meadville, US
• Chuan-Che Wang · Ithaca, US
• Po Ki Yuen · Corning, US

Key dates

• Filing date: Apr 7, 2005
• Grant date: Jan 18, 2011
• Expiry date: Jan 22, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/253
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention includes several methods for modifying the current processes of manufacturing optical sensing microplates that use continuous waveguide films to reduce/eliminate crosstalk between the biosensors that are incorporated within wells. The methods include (1) physically deteriorating/removing the waveguide film between individual biosensors; (2) chemically depositing highly absorbing materials within the waveguide film between individual biosensors; (3) patterning disordered (scattering) regions between the diffraction gratings that define individual biosensors; (4) using a specific mask and depositing individual patches of waveguide film, where each patch defines at least one biosensor. Each of these methods and several other methods described herein prevent the propagation of light between individual sensing regions, thereby eliminating optical crosstalk between the biosensors. The present invention also includes the resulting microplate.